Abstract
This study was to evaluate the effect of a preparation of Bacillus probiotic (Bacillus licheniformis and B. subtilis, 1:1) on growth and survival rate of Pacific white shrimp, Litopenaeus vannamei larvae. The larvae were fed on Artemia urmiana nauplii and Brachionus plicatilis enriched with the probiotic preparation at 19 106CFU mL-1 rate. The experimental setup was completely randomized design comprised of six treatments, namely solo Artemia nauplii (A) or rotifer (R), Artemia nauplii and rotifer without any enrichment (A? R), Artemia nauplii enrichment with probiotic bacilli (Bacillus licheniformis and B. subtilis)(A? B), rotifer enrichment with probiotic bacilli (R? B) and enriched Artemia nau-plii and rotifer (A?R? B). All treatments were per-formed in triplicate. Chemical parameters of rearing water viz. pH, salinity and temperature were 7.5–8, 30–31 ppt and 31–32 °C, respectively. Photoperiod was 16L:8D. Shrimp larvae were fedArtemia nauplii and rotifers at 5–20 and 10–40 individuals per shrimp larvae four times a day, respectively. Growth and survival rate of larvae were de-termined at MII,MIII,PL1,PL4,PL7 and PL10 stages. Larvae in A?R? B treatment showed the highest total length (10.89± 0.51 mm), weight (674± 73 lg) and survival rate (65 %± 3.5). Lowest total length, weight and survival rate (7.96± 0.63 mm, 493± 52 lg and 24.5± 2.4 %, respectively) were recorded in treatment B larvae. We concluded that Bacillus probiotic can improve growth and survival rate of Pacific white shrimp larvae without conceivably undesirable effects.
Key words
Bacillus, Shrimp, Litopenaeus vannamei, Artemia urmiana, Brachionus plicatilis
Authors
Hadi Jamali, Ahmad Imani, Daruosh Abdollahi, Reza Roozbehfar, Amin Isari
Publication
Published online: 27 February 2015. Springer Science+Business Media
